Dual color encoded Multiplex Beads

The spectral characteristics of the dyes used for the dual colour encoded system are:

Color 1:	Excitation 420 – 480 nm	Color 2:	Excitation 515 – 540 nm
	Emission 485 – 540 nm		Emission 535 – 570 nm

Dual color encoded have been developed specifically for read-out using a fluorescence microscope in combination with a suitable pattern recognition software.

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Category: Micro- and Nanoparticles (beads) Tags: KF Technology, KFT, micro, Research, Scientific Research

Description

Dual color encoded Multiplex Beads

The spectral characteristics of the dyes used for the dual colour encoded system are:

Color 1:	Excitation 420 – 480 nm	Color 2:	Excitation 515 – 540 nm
	Emission 485 – 540 nm		Emission 535 – 570 nm

Dual color encoded have been developed specifically for read-out using a fluorescence microscope in combination with a suitable pattern recognition software.

Products

Id	Mean Diameter	Color Labeling	Surface Modifications
105 12 011	11 µm	dual colour	3D-Carboxy AF
105 12 015	15 µm	dual colour	3D-Carboxy AF
105 31 011	11 µm	dual colour	Streptavidin
105 31 015	15 µm	dual colour	Streptavidin
105 32 011	11 µm	dual colour	Neutravidin
105 32 015	15 µm	dual colour	Neutravidin
105 33 011	11 µm	dual colour	3D-Alkyne
105 33 015	15 µm	dual colour	3D-Alkyne
105 34 011	11 µm	dual colour	3D-Azide
105 34 015	15 µm	dual colour	3D-Azide
105 35 011	11 µm	dual colour	Protein A/G
105 35 015	15 µm	dual colour	Protein A/G
105 36 011	11 µm	dual colour	3D-Aldehyde
105 36 015	15 µm	dual colour	3D-Aldehyde

The standard packaging volume is 1.5 mL/population with a solids content of 0.5%. Please do not hesitate to contact us, if you are interested in a set of multiplex bead populations that is tailored to your specific application and/or read-out system.

Technology for dual-color bead encoding

The definition and differentiation between bead populations used in multiplex bead assays can be achieved using a combination of fluorescence emission wavelength (colour), fluorescence intensity and bead size. This encoding principle is illustrated in the image above.

PolyAn offers both dual-colour encoding and single-dye-encoding. Dual-colour encoded beads allow a narrower definition of the bead population, but have higher requirements regarding the read-out software.

By combining fluorescence encoding and different particle size populations up to 100 populations can be defined. Using size as well as fluorescence makes it easier to differentiate between populations. The overall system becomes more robust and the requirements with regards to the read-out system can be reduced.

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